Abnormal Prion Protein [Presence] in Cerebral spinal fluid by RT-QuIC

Abn Prion Prot CSF Ql RT-QuIC

Definition

Detection of pathologic prions in cerebrospinal fluid (CSF). The test uses recombinant prion protein (PrP) as a reagent substrate and patient CSF in a real-time quaking-induced conversion (RT-QuIC) assay. If pathologic prions are present in the patient's CSF, they propagate misfolding of the reagent PrP to form amyloid, which is detected using a fluorescent indicator. RT-QuIC has been adopted in clinical practice due to the sensitivity, specificity, and use of CSF (not a brain biopsy) for testing. The reported sensitivity of RT-QuIC in detecting CJD is >90%, and the specificity approaches 100%.

abn; Abn Prion Prot; abnorm; Bovine spongiform encephalopathy; BSE; Cerebral spinal fluid; Cerebrospinal Fl; Chemistry; Ordinal; Point in time; PR; Prot; PrP; QL; Qual; Qualitative; Random; Scrapie; Screen; Spinal Fl; Spinal Fld; Spinal Flu; Spinal Fluid