PhenX - fasting serum insulin protocol 141301

Fasting serum insulin proto

Definition

This protocol provides instructions for drawing, processing and storing blood according to the National Health and Nutrition Examination Survey (NHANES) methods. As there are no standard assays for insulin, the protocol also provides basic guidelines to aid comparability among different studies. Although all three are valuable standalone measures and all should be collected if resources are available, the Diabetes Working Group recommends that investigators prioritize Insulin 1st, C-peptide 2nd and Proinsulin 3rd. This is because results from the Insulin bioassay can be combined with results from Fasting Plasma Glucose bioassay to derive the homeostatic model assessment (HOMA) of insulin resistance and pancreatic beta cell function. The Diabetes Working Group notes that while it is not the preferred method, the Serum Insulin Assay can be performed on individuals who have not met the fasting requirements. The Diabetes Working Group notes that there are also protocols to collect samples for Serum Insulin concentrations at 0, 30, 60, 90 and 120 minutes and that this could be done at the same time as the Oral Glucose Tolerance Test. Such protocols would give extra specificity and information but would increase the burden on both the participant and investigator.

HUM; Humulin; IH7; Insul; Lente; NPH; Pan; Panel; PANEL.PHENX; Panl; Pnl; Point in time; Random; Semilente; Ser; Sliding; SR; Ultralente