Entamoeba histolytica+Entamoeba dispar+Entamoeba ecuadoriensis+Entamoeba nuttalli DNA [Presence] in Specimen by NAA with probe detection

E his+dis+ecua+nutt DNA Spec Ql NAA+prb

Definition

This code describes molecular detection of Entamoeba histolytica/dispar complex (E. histolytica, E. dispar, E. nuttalli, and E ecuadoriensis) DNA by RT-PCR. It targets a sequence of the DNA coding for the 15S ribosomal RNA that is common to these four species. So a positive result indicates whether any one of these species is present without specifying which one. In the submitter's laboratory, it is used only on human specimens, mostly stool but pus and tissue as well. E. dispar is a commensal in the human digestive tract, and E. histolytica is the only one thought to be pathogenic in humans. The other two species are found in animals and not thought to inhabit the human gut. A positive can be followed up in the submitter's lab by specific identification of the species using DNA sequencing (see LOINC 70294-4). Additional species that have been found in the gut include Entamoeba moshkovskii, Entamoeba poleki, Entamoeba coli, and Entamoeba hartmanni. E. histolytica, E. dispar, and E.moshkovskii are morphologically identical but are different biochemically and genetically.

Entamoeba histolytica+Entamoeba dispar+Entamoeba ecuadoriensis+Entamoeba nuttalli DNA

3 Self-Sustaining Sequence Replication; 3SR SR; Ameba; Amebiasis; Amebic; Amoeba; Amplif; Amplification; Amplified; Deoxyribonucleic acid; DNA NUCLEIC ACID PROBE; DNA probe; E dispar; E his+dis+ecua+nutt; E histolyt; E histolytica; E histolytica dispar complex; Entamoeba histolytica dispar complex; Entamoeba histolytica/dispar complex; ID; Infectious Disease; InfectiousDisease; LAT; LCR; Ligase chain reaction; Ligation-activated transcription; Microbiology; Misc; Miscellaneous; NAA+probe; NAAT; NASBA; Nucleic acid sequence based analysis; Ordinal; Other; PCR; Point in time; Polymerase chain reaction; PR; Probe amp; Probe with ampification; Probe with target amplification; QBR; QL; Qual; Qualitative; Random; Screen; SDA; Spec; Strand Displacement Amplification; TMA; To be specified in another part of the message; Transcription mediated amplification; Unspecified

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