NPM1 gene c.956dupTCTG transcript/control transcript [# Ratio] in Blood or Tissue by Molecular genetics method

NPM1 gene mut A/control Bld/T

Definition

The quantification of NPM1 mutation A (c.956dupTCTG) transcripts are compared to a control gene (e.g. ABL1) by real-time PCR (RT-qPCR). NPM1 primers and probes spanning exons 11 and 12 may be used to detect quantify the NPM1 mutation transcripts. Results are based on the normalized copy number [NPM1 mut D copy number/ABL1 copy number) x 100]. This test is based on, but not limited to, IPSOGEN'S NPM1-A MutaQuant Kit, which is used as research tool (as of 2012) for the quantification of NPM1 transcripts in samples previously identified as carrying the NPM1 mutation A. The results are used to monitor the decrease of NPM1 mutA expression after treatment.

ABL; ABL1; ABL1 transcript; B23; Blood; control gene; Genetics; Heredity; Heritable; Inherited; Molecular genetics; Molecular pathology; MOLPATH; MOLPATH.MUTATIONS; NPM; NPM1 gene mut A; Nucleolar phosphoprotein b23; nucleophosmin (nucleolar phosphoprotein B23, numatrin); Numatrin; Number ratio; PCR; Point in time; QNT; Quan; Quant; Quantitative; Random; Tissue; Tissue, unspecified; WB; Whole blood; Whole blood or Tissue