NPM1 gene c.960insCCTG transcript/control transcript [# Ratio] in Bone marrow by Molecular genetics method

NPM1 gene mut D/control Mar

Definition

The quantification of NPM1 mutation D (c.960insCCTG) transcripts are compared to a control gene (e.g. ABL1) by real-time PCR (RT-qPCR). NPM1 primers and probes spanning exons 11 and 12 may be used to detect quantify the NPM1 mutation transcripts. Results are based on the normalized copy number [NPM1 mut D copy number/ABL1 copy number) x 100]. This test is based on, but not limited to, IPSOGEN'S NPM1-B/D MutaQuant Kit, which is used as research tool (as of 2012) for the quantification of NPM1-D transcripts in samples previously identified as carrying the NPM1 mutation D. The results are used to monitor the decrease of NPM1 mutD expression.

ABL; ABL1; ABL1 transcript; B23; BM; BON; Bone marrow; control gene; Genetics; Heredity; Heritable; Inherited; MAR; Marrow (bone); minimal angle of resolution; Minimum angle of resolution; Molecular genetics; Molecular pathology; MOLPATH; MOLPATH.MUTATIONS; NPM; NPM1 gene mut D; Nucleolar phosphoprotein b23; nucleophosmin (nucleolar phosphoprotein B23, numatrin); Numatrin; Number ratio; PCR; Point in time; QNT; Quan; Quant; Quantitative; Random