Mycobacterium tuberculosis stimulated gamma interferon and spot count panel - Blood

M TB IFN-g+spot Pnl Bld

Definition

Interferon gamma release assays (IGRAs) are used for the detection of M. tuberculosis. This panel was created for, but not limited in use to, Oxford Immunotec's T-SPOT.TB assay, which uses enzyme-linked immunospot (ELISPOT) methodology to enumerate M. tuberculosis-sensitized T cells by capturing interferon-gamma (IFN-y) in the vicinity of T cells in the patient's whole blood. Cells are spun and, using the ficoll centrifugation procedure, the polys and RBCs layer together in the bottom fraction with peripheral blood mononuclear cells (PBMCs) in the middle. The PBMCs are pipetted out to yield about 250,000 PBMCs per aliquot. They are incubated with M. tuberculosis specific antigens (ESAT-6, CFP10) in two separate wells, a phytohemagglutinin (PHA) control (a mitogenic stimulator) in another well, and a negative control in a fourth well. Incubation with the antigens allows for stimulation of any sensitized T cells present. Evaluating the number of antigen incubated spots compared to the positive and negative control spots provides a measurement of the abundance of M. tuberculosis sensitive T cells in the patient's blood.

Acid Fast; Acid Fast Bacilli; Acid fast bacillus; AFB; Blood; ELISPOT; ID; IFN; IGRA; Infectious Disease; InfectiousDisease; M tb; M TB IFN-g; M TB IFN-g+spot Pnl; M tuberculosis; Microbiology; Mtb; Myco; Mycobact; Mycobacterium tuberculosum; Pan; PANEL.MICROBIOLOGY; Panl; Pnl; Point in time; Random; TB; T-SPOT; T-SPOT.TB; Tuberculosis; WB; Whole blood