Blood group antibody screen [Presence] in Serum or Plasma by GEL

Bld gp Ab Scn SerPl Ql GEL

Definition

Antibody screening is used to demonstrate unexpected antibodies (other than ABO) and is performed on both donors and recipients to help ensure compatibility between recipient antibodies and donor red cells. GEL antibody testing (column agglutination technology) is an alternative to tube testing and uses a microtube with a reaction chamber containing dextran acrylamide gel and other elements. Red cells and plasma are added to the gel column top, incubated, and spun. In the microtubes filled with gel particles and anti-IgG, the particles separate the red cell clusters physically (larger agglutinates, less migration through gel) and the anti-IgG captures the IgG coated red cells and decreases their migration through the gel immunologically. For negative results, red cells appear in a button at the bottom of the microtube. For positive results, the red cells are stopped in areas throughout the microtube; the more positive the reaction, the higher the position in the tube. GEL testing can also be done without IgG in the gel (for ABO testing for example). Sensitivity is similar to PEG testing. GEL testing can be automated or performed manually.

Ab; Bld gp Ab Scn; BLOOD BANK; Ordinal; Pl; Plasma; Plsm; Point in time; PR; QL; Qual; Qualitative; Random; Scn; Scr; Screen; Scrn; SerP; SerPl; SerPlas; Serum; Serum or plasma; SR