Blood group antibody screen [Presence] in Serum or Plasma by Low ionic strength saline (LISS)

Bld gp Ab Scn SerPl Ql LISS

Definition

Antibody screening is used to demonstrate unexpected antibodies (other than ABO) and is performed on both donors and recipients to help ensure compatibility between recipient antibodies and donor red cells. Low ionic strength saline (LISS) is a blood banking potentiating agent that works by decreasing the repelling charges between red cells (zeta potential), thus packing the reds cells closer together for increased antibody bridging. This then decreases the incubation time for the sensitization phase in the antibody screening. A downside to using LISS is that it enhances the expression of cold antibodies and auto-antibodies that may not be significant. In addition, the ratio of serum to red cells must be adhered to strictly to maintain the ionic strength of the mixture. If ionic strength is too high, false negatives may occur, and if it is too low, false positives due to complement binding may occur.

Ab; Bld gp Ab Scn; BLOOD BANK; Low ionic strength saline; Ordinal; Pl; Plasma; Plsm; Point in time; PR; QL; Qual; Qualitative; Random; Scn; Scr; Screen; Scrn; SerP; SerPl; SerPlas; Serum; Serum or plasma; SR