Yellow fever virus ns5 gene [Presence] in Cerebral spinal fluid by NAA with non-probe detection

YFV ns5 gene CSF Ql NAA+n-prb

Definition

This assay comprises qRT-PCR targeting the ns5 gene in the yellow fever backbone followed by melt curve analysis, which identifies DNA fragments with different nucleotide compositions based on their unique melting temperature. [PMID: 18501437] CSF testing is indicated for suspected adverse neurological reactions to the vaccine against yellow fever. Genome detection of CSF yellow fever vaccine in this context is considered diagnostic. In suspected patients, detection of antibodies against yellow fever in CSF should also be requested.

3 Self-Sustaining Sequence Replication; 3SR SR; American plague; Amplif; Amplification; Amplified; Cerebral spinal fluid; Cerebrospinal Fl; DNA NUCLEIC ACID PROBE; DNA probe; ID; Infectious Disease; InfectiousDisease; LAT; LCR; Ligase chain reaction; Ligation-activated transcription; Microbiology; NAA+n-prb; NAA+probe; NAAT; NASBA; Neuro; Neurology; Nucleic acid sequence based analysis; Ordinal; PCR; Point in time; Polymerase chain reaction; PR; Probe amp; Probe with ampification; Probe with target amplification; QBR; QL; Qual; Qualitative; Random; Screen; SDA; Spinal Fl; Spinal Fld; Spinal Flu; Spinal Fluid; Strand Displacement Amplification; TMA; Transcription mediated amplification; yellow jack; YFV; YFV ns5 gene

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